大量高分文章單細(xì)胞轉(zhuǎn)錄組研究策略及10x 合作項(xiàng)目部分結(jié)果展示

發(fā)稿時(shí)間：2019-04-18來(lái)源：天昊生物

10x研究介紹

單細(xì)胞轉(zhuǎn)錄組分析（scRNA-seq）是一項(xiàng)相當(dāng)年輕的技術(shù)，也是目前最熱點(diǎn)的研究方向之一。10x Genomics單細(xì)胞轉(zhuǎn)錄組測(cè)序是基于油包水液滴進(jìn)行單細(xì)胞分離，并針對(duì)單個(gè)細(xì)胞內(nèi)的mRNA 進(jìn)行擴(kuò)增和高通量測(cè)序的一項(xiàng)新技術(shù)。單細(xì)胞表達(dá)譜的繪制能夠完美地揭示細(xì)胞間基因表達(dá)的異質(zhì)性，從復(fù)雜生物樣本中發(fā)現(xiàn)稀有細(xì)胞類(lèi)型。10x Genomics單細(xì)胞轉(zhuǎn)錄組測(cè)序適用于絕大部分細(xì)胞類(lèi)型，并且被廣泛應(yīng)用于腫瘤免疫、腫瘤靶向治療、腫瘤微環(huán)境、非腫瘤相關(guān)復(fù)雜疾病、發(fā)育與細(xì)胞分化及植物研究等方向。2019年以來(lái)，10x Genomics單細(xì)胞轉(zhuǎn)錄組測(cè)序已經(jīng)發(fā)表近百篇文章，10分以上有60%之多。前沿領(lǐng)域，需要的是馬上行動(dòng)起來(lái)！看看下面的研究思路是不是能為你帶來(lái)新的提示呢？

 

單細(xì)胞轉(zhuǎn)錄組研究策略

 

 

10x技術(shù)參數(shù)

 

 

10x項(xiàng)目流程

 

 

10x合作項(xiàng)目部分結(jié)果展示

 

測(cè)序數(shù)據(jù)及細(xì)胞質(zhì)控結(jié)果

 

樣本中單細(xì)胞基因數(shù)、UMI序列數(shù)及線粒體基因UMI序列比例

 

單個(gè)樣本細(xì)胞聚類(lèi)tSNE圖

 

Marker基因CD27 tSNE圖及小提琴圖

 

10x應(yīng)用領(lǐng)域

腫瘤細(xì)胞異質(zhì)性

Single-cell transcriptomes from human kidneys reveal the cellular identity of renal tumors, 2018, Science.（腎細(xì)胞癌）

Single cell analysis reveals cancer stem cell heterogeneity in hepatocellular carcinoma, 2018, Hepatology.（肝細(xì)胞癌）

腫瘤藥物反應(yīng)/靶向治療

Single-cell transcriptome analyses reveal endothelial cell heterogeneity in tumors and changes following anti-angiogenic treatment, 2018, Cancer Research.（抗血管生成）

Single-cell RNA-seq analysis identifies markers of resistance to targeted BRAF inhibitors in melanoma cell populations, 2018, Genome Research.（靶向抑制劑）

腫瘤免疫/免疫治療

Acquired cancer resistance to combination immunotherapy from transcriptional loss of class I HLA, 2018, Nature Communications.（治療進(jìn)展；血液+組織）

Arg1 expression defines immunosuppressive subsets of tumor-associated macrophages, 2018, Theranostics.（anti-PD-1；組織）

非腫瘤相關(guān)疾病

Single-cell transcriptomics of the mouse kidney reveals potential cellular targets of kidney disease, 2018, Science.（腎臟疾?。?

Single-cell RNA-seq reveals the transcriptional landscape and heterogeneity of aortic macrophages in murine atherosclerosis, 2018, Circulation Research.（動(dòng)脈粥樣硬化）

發(fā)育/分化

Single-cell RNA sequencing of microglia throughout the mouse lifespan and in the injured brain reveals complex cell-state changes, 2019, Immunity.（小神經(jīng)膠質(zhì)細(xì)胞）

Single-cell transcriptomic analysis of cardiac differentiation from human PSCs reveals HOPX-dependent cardiomyocyte maturation, 2018, Cell Stem Cell.（干細(xì)胞，心臟分化）

植物領(lǐng)域

Single-cell RNA sequencing resolves molecular relationships among individual plant cells, 2019, Plant Physiology.（擬南芥）

Spatiotemporal developmental trajectories in the arabidopsis root revealed using high-throughput single-cell RNA sequencing, 2019, Developmental Cell.（擬南芥）